Thermo Scientific™ DDM/CHS Solution (10:1)

• A ready-to-use solution for solubilization of membrane proteins while maintaining structural integrity and activity
• The stable pre-formulated solution contains a mixture of dodecyl-β-D-maltoside (DDM) (100 mg/mL) and cholesteryl hemisuccinate (CHS) (10 mg/mL) in deionized water with final concentrations of 10% and 1%, respectively
• Typical working concentrations are 1% DDM/0.1% CHS for protein extraction and 0.1% DDM/0.01% CHS for protein purification

Features of DDM/CHS Solution include:

• Ready-to-use liquid formulation
• Mixture of nonionic detergent, DDM, with dual hydrophobic/hydrophilic properties and anionic detergent, CHS, that aids in the stabilization of large unilamellar vesicles
• DDM/CHS micelles aid in solubilization of membrane proteins while preserving structure and function
• Suitable for protein solubilization and stabilization for single particle cryo-EM analysis

Properties of DDM:

• Molecular formula: C₂₄H₄₆O₁₁
• Molecular weight: 510.6 g/mol
• Detergent class: Non-ionic
• Micelle molecular weight: 50,000 g
• Critical micelle concentration (CMC): 0.17 mM (0.009% w/v) in water; 0.12 mM (0.006% w/v) in 0.2 M NaCl

Properties of CHS:

• Molecular formula: C₃₁H₅₀O₄ · C₄H₁₁NO₃
• Molecular weight: 607.9 g/mol
• Detergent: Ionizable anionic
• Function: Stabilizes large unilamellar vesicles