Applied Biosystems™ Custom Plus TaqMan™ Copy Number Assay

Applied Biosystems TaqMan Copy Number Assays use gold-standard TaqMan MGB probe chemistry to evaluate the copy number of genomic DNA targets using Applied Biosystems real-time PCR instruments and software. TaqMan Copy Number Assays are run together with a TaqMan Copy Number Reference Assay in a duplex qPCR reaction; the copy number assay detects the target sequence, and the reference assay detects a sequence that is known to be present in two copies in the diploid genome. The results are then analyzed by the relative quantitation method using Applied Biosystems CopyCaller Software.

Custom Plus TaqMan Copy Number Assays are an optimal solution for studying variation in human and mouse genomic regions of interest for which a predesigned assay is not available. Using the free GeneAssist Copy Number Assay Tool, these assays are designed with the same bioinformatics pipeline used to develop the assays in the predesigned collection. The design process for Custom Plus assays includes up-front bioinformatic sequence analysis and in silico QC.

Benefits:
• Flexible—generate designs for human and mouse targets not covered by the predesigned assay collection using the free GeneAssist Copy Number Assay Tool
• Specific—gold-standard TaqMan chemistry with minor groove binder (MGB) probes increase assay specificity by enabling shorter probes
• Reproducible—robust assay designs developed with our bioinformatics pipeline enable accurate, reproducible, and reliable results
• Easy to interpret—CopyCaller Software provides the calculated copy number and predicted copy number, along with confidence value and z-score quality metrics
• Fast and simple—setup to primary analysis in 3–4 hours

Approximate ship time
4–6 days in North America and 6–10 days in Europe

All assay designs are the product of our industry-leading bioinformatics pipeline, optimized over the course of more than a decade by leveraging manufacturing and assay performance data. TaqMan Assays have been cited in over 40,000 publications and are backed by more than 350 patents.

TaqMan Copy Number Reference Assays are sold separately.

Recommended master mix (sold separately): TaqMan Genotyping Master Mix (Cat. No. 4371355)