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Descrizione
ExpiCHO Expression Medium is specifically designed for high-density growth and transfection of suspension-adapted Chinese hamster ovary (CHO) cells. This medium is GMP-manufactured, chemically-defined, serum-free, protein-free, and animal origin–free. It is a core component of the ExpiCHO Expression System Kit (Cat. No. A29133) for scalable transient protein production. To achieve the highest levels of transient protein expression, the medium is matched to a culture feed, ExpiCHO Feed, also included in the system kit and transfection kits.
With ExpiCHO Expression Medium you can:
- Grow suspension CHO cultures to densities of over 2 x 107 cells per mL, with >95% cell viability
- Achieve high transfection efficiencies of >90% when coupled with ExpiFectamine CHO transfection reagent
- Transiently transfect suspension CHO cells at a density of 6 x 106 cells per mL
- Achieve yields up to 3 g per L of recombinant protein of high-density transiently transfected cultures by sustained, high-level expression
- Grow and transfect CHO cells at volumes of <1 mL up to 5 L in culture formats from multiwell plates up to 5 L shake flask
ExpiCHO Expression Medium is ready to use. It is formulated with GlutaMAX-I reagent and does not require supplementation. It does not contain phenol red. The chemically-defined, animal origin free formulation results in high reproducibility and lot-to-lot reliability. ExpiCHO Expression Medium is not recommended for adherent cell cultures.
Specifica
Specifica
| Linea cellulare | ExpiCHO-S™, CHO-S, SFM Adapted |
| Tipo di cellula | Chinese hamster ovary (CHO) cells |
| Classificazione | Animal Origin-free, Chemically-defined, Protein-free, Serum-free |
| Concentrazione | 1X |
| Forma | Liquid |
| Product Type | CHO Expression Medium |
| Serum Level | Serum-free |
| Sterilità | Sterile |
| Sterilization Method | Sterile-filtered |
| Con additivi | GlutaMAX |
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