Gibco™ CO₂ Independent Medium

• CO₂ Independent Medium is a non-HEPES proprietary medium used for supporting cell growth for a variety of suspension and adherent mammalian cells such as epithelial, fibroblast, and lymphoid cell lines without a CO₂ incubator
• CO₂ Independent Medium is ideal for transporting cells or tissue, and for the handling of mouse embryos under atmospheric conditions
• Gibco™ CO₂ Independent Medium contains a unique buffering system composed of mono and dibasic sodium phosphate and β-glycerophosphate
• A small amount of sodium bicarbonate has been included in the formulation to meet essential bicarbonate dependent functions
• No synthetic buffers are utilized, thus eliminating any cytotoxic effects associated with such buffering systems
• Additionally, Gibco™ CO₂ Independent Medium has been formulated with components that enhance cellular production and utilization of CO₂
• Store at 2°C to 8°C, protected from light

Modified as follows

• With: phenol red, sodium pyruvate
• Without: L-glutamine, HEPES

Using CO₂ Independent Medium

• CO₂ Independent Medium contains no lipids or growth factors therefore it requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
• The CO₂ Independent Medium does not require a CO₂ environment to maintain physiological pH
• For maximum growth performance some cell lines may require either direct or sequential adaptation to CO₂ Independent Medium