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Thermo Scientific Chemicals Citrat, 0.5 M-Pufferlösung, pH 3.5

Product Code. 15464239
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Product Code. 15464239 Supplier Thermo Scientific Chemicals Supplier No. J61690.AP
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CAS: 6132-04-3294.10 g/mol

Citrate, is used as an anticoagulant and also used as a biological buffer. It is often used to prepare sodium citrate buffer for antigen retrieval of tissue samples. The citrate solution is designed to break protein cross-links, thus, unmasking antigens and epitopes in formalin-fixed and paraffin embedded tissue sections, resulting in enhancing staining intensity of antibodies. Citrate has anticoagulant activity and as a calcium chelator, it forms complexes that disrupt the tendency of blood to clot.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Anwendungen
Citrat, wird als Antikoagulans und auch als biologischer Puffer verwendet. Es wird häufig zur Vorbereitung von Natriumcitratpuffer für die Antigengewinnung von Gewebeproben verwendet. Die Citrat-Lösung wurde entwickelt, um Protein-Querverbindungen zu brechen. Daher werden Antigene und Epitope in formalinfixierten und paraffineingebetteten Gewebeschnitten abgetastet, was zu einer Erhöhung der Färbungsintensität von Antikörpern führt. Citrat weist antikoagulante Aktivität auf und bildet als Kalziumchelator Komplexe, die verhindern, dass das Blut gerinnt.

Löslichkeit
Löslich in Wasser (29.4 g/l) bei 20 °C. Unlöslich in Ethanol.

Hinweise
Kühl lagern. Behälter an einem trockenen und gut belüfteten Ort dicht verschlossen halten. Von starken Oxidationsmitteln fernhalten.
TRUSTED_SUSTAINABILITY

Chemical Identifiers

CAS 4-3-6132
Molekulargewicht (g/mol) 294,10

Specifications

Farbe Farblos
Konzentration 0,5 mM Pufferlösung
pH 3,5
Physikalische Form Flüssig
Menge 500 ml
Geruch Geruchsneutral

RUO – Research Use Only

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