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CD8a, Super Bright 702, clone: 53-6.7, eBioscience™

Rat Monoclonal Antibody

Marke:  Invitrogen 67-0081-80

Additional Details : Gewicht : 0.01000kg

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Artikelnummer. 15805713

  • 184.00 CHF / 25 Mikrogramm


Geschätzter Ausliefertermin
von Anbieter 31-01-2019
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Beschreibung

Beschreibung

Description: The 53-6.7 monoclonal antibody reacts with the mouse CD8a molecule. CD8a is an approximately 32-34kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha beta) or as a homodimer (CD8 alpha alpha). A majority of thymocytes and a subpopulation of mature alpha beta TCR T cells express CD8 alpha beta while gamma delta TCR T cells, a subpopulation of intestinal intraepithelial lymphocytes (IELs) and dendritic cells express CD8 alpha alpha. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells.Applications Reported: This 53-6.7 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 53-6.7 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Super Bright 702 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 702 nm. We recommend using a 710/50 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome. When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (cat. SB-4400) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100μL of cell sample + 100μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 405 nm; Emission: 702 nm; Laser: Violet Laser
Spezifikation

Spezifikation

CD8a
Monoclonal
0.2mg/mL
PBS with BSA and 0.09% sodium azide; pH 7.2
P01731
CD8A
IgG2a, kappa
25μg
4°C, store in dark, DO NOT FREEZE!
Primary
12525
Flow Cytometry
53-6.7
Super Bright 702
Cd8a
Liquid
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse
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