missing translation for 'onlineSavingsMsg'
Learn More

CD8a, Super Bright 600, clone: RPA-T8, eBioscience™

Mouse Monoclonal Antibody

Marke:  Invitrogen 63-0088-41

Additional Details : Gewicht : 0.01000kg

 Lernen Sie weitere Varianten dieses

Artikelnummer. 15805553

  • 180.00 CHF / 25 Stück


Geschätzter Ausliefertermin
von Anbieter 25-01-2019
Zum Warenkorb hinzufügen

Beschreibung

Beschreibung

Description: The RPA-T8 monoclonal antibody reacts with the human CD8a molecule, an approximately 32-34kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha/beta) or as a homodimer (CD8 alpha/alpha). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T-cell development and activation of mature T cells.Applications Reported: This RPA-T8 antibody has been reported for use in flow cytometric analysis.Applications Tested: This RPA-T8 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5μL (0.125μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Super Bright 600 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 600 nm. We recommend using a 610/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (cat. SB-4400) to minimize any non-specific polymer interactions. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100μL of cell sample + 100μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 2-8°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorochrome performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 405 nm; Emission: 600 nm; Laser: Violet Laser
Spezifikation

Spezifikation

CD8a
Monoclonal
5μl/Test
PBS with BSA and 0.09% sodium azide; pH 7.2
P01732
CD8 alpha, leu-2a
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
RPA-T8
Super Bright 600
CD8A
Liquid
CD8A
IgG1, kappa
25 Tests
4°C, store in dark, DO NOT FREEZE!
Primary
925
Videos

For Research Use Only