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CD80 (B7-1) Armenian Hamster anti-Canine, Mouse, Porcine, Super Bright 600, Clone: 16-10A1, eBioscience™

Armenian Hamster Monoclonal Antibody

Marke:  Invitrogen 63-0801-82

Additional Details : Gewicht : 0.01000kg

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Artikelnummer. 15825583

  • 400.00 CHF / 100 Mikrogramm


Verfügbar ab
26-05-2021
Zum Warenkorb hinzufügen

Beschreibung

Beschreibung

Description: The 16-10A1 monoclonal antibody reacts with mouse CD80 (B7-1), a 55 kDa member of the Ig superfamily. CD80 is expressed by macrophages, dendritic cells and activated B cells. In addition, activated T cells express this antigen. CD80 has high affinity for binding to two T cell surface antigens, CD28 and CD152 (CTLA-4). The interaction of CD28 and CD152 with CD80 is crucial in T-B cell communication leading to activation of T and B cells, respectively. The 16-10A1 monoclonal antibody crossreacts with pig and dog. Applications Reported: This 16-10A1 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 16-10A1 antibody has been tested by flow cytometric analysis of stimulated mouse splenocytes. This can be used at less than or equal to 0.03 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Super Bright 600 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 600 nm. We recommend using a 610/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.

When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product No. SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 2-8°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorochrome performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 405 nm; Emission: 600 nm; Laser: Violet Laser Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company. CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28 and of an inhibitory receptor CTLA-4 (CD152). The both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, the both molecules can also substitute for each other in this process. The question what are the differences in CD80 and CD86 competency has not been fully elucidated yet; there are still conflicts in results about their respective roles in initiation or sustaining of the T cell immune response.
Spezifikation

Spezifikation

CD80 (B7-1)
Monoclonal
0.2 mg/mL
PBS with BSA and 0.09% sodium azide; pH 7.2
Q00609
CD80
IgG
100 μg
4° C, store in dark, DO NOT FREEZE!
Primary
12519, 397161, 403765
Flow Cytometry
16-10A1
Super Bright 600
Cd80
Liquid
Armenian Hamster
Affinity chromatography
RUO
Antibody
Monoclonal
Canine, Mouse, Porcine
Videos
Sicherheitsdatenblatt (SDS)
Dokumentation

For Research Use Only