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CD73 Monoclonal Antibody (AD2), Super Bright™ 702, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
163.00 CHF - 376.00 CHF
Spezifikation
Antigen | CD73 |
---|---|
Klon | AD2 |
Konzentration | 5 μL/Test |
Anwendungen | Flow Cytometry |
Klassifikation | Monoclonal |
Produktcode | Marke | Menge | Preis | Menge & Verfügbarkeit | |||||
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Produktcode | Marke | Menge | Preis | Menge & Verfügbarkeit | |||||
15973734
|
Affymetrix eBioscience
67-0739-42 |
100 Tests |
376.00 CHF
100 Stück |
Verfügbar ab: 08-05-2024
Loggen Sie sich ein, um den Lagerbestand zu sehen |
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15963734
|
Affymetrix eBioscience
67-0739-41 |
25 Tests |
Dieser Artikel wurde eingestellt und ist leider nicht mehr verfügbar. Sehen Sie sich das Produkt an, um alternative Produktvorschläge zu sehen oder kontaktieren Sie unseren Technischen Support unter 056 618 41 11. |
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Beschreibung
Description: This AD2 monoclonal antibody reacts with human CD73, a 5'-ectonucleotidase that converts 5'-adenosine monophosphate to adenosine. CD73 is expressed on the surface of endothelial cells, as well as B and T cells, including some CD4+Foxp3+ regulatory T cells. Adenosine production by these cells has been linked to the inhibition of CD4 T cell effector functions such as proliferation and cytokine secretion.
Applications Reported: This AD2 antibody has been reported for use in flow cytometric analysis. Applications Tested: This AD2 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Super Bright 702 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 702 nm. We recommend using a 710/50 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome. When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 405 nm; Emission: 702 nm; Laser: Violet Laser Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.Spezifikation
CD73 | |
5 μL/Test | |
Monoclonal | |
Liquid | |
RUO | |
P21589 | |
NT5E | |
Primary | |
4° C, store in dark, DO NOT FREEZE! | |
NT5E |
AD2 | |
Flow Cytometry | |
Super Bright 702 | |
Mouse | |
Human | |
4907 | |
IgG1 κ | |
Affinity chromatography | |
Antibody |