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CD69, Super Bright 600, clone: H1.2F3, eBioscience™

Armenian Hamster Monoclonal Antibody

Marke:  Invitrogen 63-0691-80

Additional Details : Gewicht : 0.01000kg

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Artikelnummer. 15805583

  • 159.00 CHF / 25 Mikrogramm


Geschätzter Ausliefertermin
von Anbieter 31-01-2019
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Beschreibung

Beschreibung

Description: The H1.2F3 monoclonal antibody reacts with mouse CD69, also known as very early activation antigen (VEA). CD69 is approximately 35 kDa and is expressed on the surface as a disulfide-linked dimer. While a small subset of lymphocytes in the thymus, spleen and lymph nodes express this antigen, activation of both T and B cells rapidly upregulates the surface expression of CD69, suggesting a role for CD69 in lymphocyte development and activation.Applications Reported: This H1.2F3 antibody has been reported for use in flow cytometric analysis.Applications Tested: This H1.2F3 antibody has been tested by flow cytometric analysis of stimulated mouse splenocytes. This may be used at less than or equal to 0.25μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Super Bright 600 is a tandem dye that can be excited with the violet laser line (405nm) and emits at 600nm. We recommend using a 610/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (Product # SB-4400) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100μL of cell sample + 100μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 405nm; Emission: 600nm; Laser: Violet Laser
Spezifikation

Spezifikation

CD69
Monoclonal
0.2mg/mL
PBS with BSA and 0.09% sodium azide; pH 7.2
P37217
5830438K24Rik; AI452015; AIM; Cd69; VEA
Armenian Hamster
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse
Flow Cytometry
H1.2F3
Super Bright 600
Cd69
Liquid
Cd69
IgG
25μg
4°C, store in dark, DO NOT FREEZE!
Primary
12515
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