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CD45, Super Bright 645, clone: 2D1, eBioscience™

Mouse Monoclonal Antibody

Marke:  Invitrogen 64-9459-42

Additional Details : Gewicht : 0.01000kg

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Artikelnummer. 15815703

  • 389.00 CHF / 100 Stück


Geschätzter Ausliefertermin
von Anbieter 31-01-2019
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Beschreibung

Beschreibung

Description: The 2D1 monoclonal antibody reacts with all isoforms of human CD45, also known as Leukocyte Common Antigen (LCA). CD45 is expressed by all hematopoietic cells excluding circulating erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in activation of lymphocytes.Applications Reported: This 2D1 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 2D1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5μL (0.5μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Super Bright 645 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 645 nm. We recommend using a 660/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (cat. SB-4400) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100μL of cell sample + 100μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 405 nm; Emission: 645 nm; Laser: Violet Laser
Spezifikation

Spezifikation

CD45
Monoclonal
5μl/Test
PBS with BSA and 0.09% sodium azide; pH 7.2
P08575
PTPRC
IgG1, kappa
100 Tests
4°C, store in dark, DO NOT FREEZE!
Primary
5788
Flow Cytometry
2D1
Super Bright 645
PTPRC
Liquid
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Human
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