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Invitrogen™ CD4 Monoclonal Antibody (N1UG0), Alexa Fluor™ Plus 594

Artikelnummer. 30202506
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Menge:
100 μg
Packungsgröße:
100 Mikrogramm
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30202506 100 μg 100 Mikrogramm
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Artikelnummer. 30202506 Lieferant Invitrogen™ Lieferanten-Nr. 755244482

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Beschreibung

Mouse Monoclonal Antibody

Description: The N1UG0 monoclonal antibody reacts with human CD4, a 59 kDa cell surface glycoprotein expressed by the majority of thymocytes, a subpopulation of mature T cells (T-helper cells) and in low levels on monocytes. CD4 is a receptor for the human immunodeficiency virus (HIV). The N1UG0 antibody is recommended for use in staining human formalin-fixed paraffin embedded tissue sections. Applications Reported: This N1UG0 antibody has been reported for use in flow cytometric analysis, western blotting, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections. Applications Tested: This N1UG0 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using high pH antigen retrieval and can be used at 20 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.

The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].
TRUSTED_SUSTAINABILITY

Spezifikation

Antigen CD4
Anwendungen Immunohistochemistry (Paraffin), Multiplex Immunohistochemistry
Klassifikation Monoclonal
Klon N1UG0
Konzentration 0.2 mg/mL
Konjugat Alexa Fluor Plus 594
Zusammensetzung PBS with 0.5% BSA, 10% proprietary stabilizer and 0.05% sodium azide; pH 7.2
Gen CD4
Gen-Zugriffsnummer P01730
Gen-Alias Activation B7-1 antigen; B7; B7.1; B7-1; BB1; B-lymphocyte activation antigen B7; CD28LG; CD28LG1; CD4; CD4 antigen; CD4 antigen (p55); CD4 antigen p55; Cd4 molecule; CD4 precursor; CD4 receptor; CD4, allele 1; cd4a; CD4mut; CD80; CD80 antigen (CD28 antigen ligand 1, B7-1 antigen); CD80 molecule; cell surface glycoprotein CD4; costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CTLA-4 counter-receptor B7.1; fCD4; L3T4; LAB7; Leu-3; Ly-4; lymphocyte antigen CD4; lymphocyte antigen CD4 precursor; membrane protein; p55; T-cell differentiation antigen L3T4; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4; T-cell surface glycoprotein CD4 precursor (T-cell surface antigen T4/Leu-3) (T-cell differentiation antigen L3T4); T-lymphocyte activation antigen CD80; W3/25; W3/25 antigen
Gensymbole CD4
Wirtsspezies Mouse
Reinigungsverfahren Affinity chromatography
Menge 100 μg
Regulatorischer Status RUO
Primär oder sekundär Primary
Gen-ID (Entrez) 920
Zielspezies Human
Inhalt und Lagerung 4°C, store in dark
Produkttyp Antibody
Form Liquid
Isotype IgG2b κ
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