Learn More
CD366 (TIM3) Monoclonal Antibody (F38-2E2), Super Bright™ 645, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
Marke: Affymetrix eBioscience 64-3109-42
Additional Details : Gewicht : 0.01000kg
Beschreibung
Description: The monoclonal antibody 29A1.4 recognizes mouse NKp46, also known as CD335. CD335, a member of the natural cytotoxicity receptor (NCR) family, is a glycoprotein with 2 Ig-like domains and a short cytoplasmic tail. Expression of CD335 is uniquely found on NK cells (including immature NK cells, defined as DX5- CD3-, and thereby allowing discrimination between NKT cells and NK cells (NKp46+, CD3-). Furthermore, unlike many of the NK markers which also stain NKT cells, staining with 29A1.4 is not strain specific. Staining has been shown on C57Bl/6, SJL, CBA/CA and BALB/C strains. NKp46 has been shown to play a role in NK cell-mediated lysis of several tumor cells and pathogen-infected cell lines. The 29A1.4 monoclonal antibody has been shown to activate NK cells in vitro. The 29A1.4 monoclonal antibody does not deplete NK cells in vivo. Applications Reported: This 29A1.4 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 29A1.4 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
TIM3 (Hepatitis A virus cellular receptor 2, HAVCR2, T-cell immunoglobulin, mucin-dmain containing-3) is a 281 amino acid long, Type-1 Th1- specific cell surface glycoprotein expressed on terminally differentiated CD4+Th1 and CD8+Tc1 cells. TIM3 consists of an IgV-like domain, a mucin-like domain in the extracellular region, and a conserved Tyrosine phosphorylation motif in the cytoplasmic region. TIM3 is involved in macrophage activation and induction of autoimmune diseases. Further, TIM3 down-regulates aggressive Th1-mediated immune responses and facilitates in the development of immune tolerance. Pathological significance of TIM3 has been attributed to Experimental autoimmune encephalomyelitis (EAE), a Th-1 dependent autoimmune disease, and also enhances the severity of experimental autoimmune encephalomyelitis in mice.Spezifikation
CD366 (TIM3) | |
Monoclonal | |
5 μL/Test | |
PBS with BSA and 0.09% sodium azide; pH 7.2 | |
Q8TDQ0 | |
Havcr2 | |
Affinity chromatography | |
RUO | |
84868 | |
4° C, store in dark, DO NOT FREEZE! | |
Liquid |
Flow Cytometry | |
F38-2E2 | |
Super Bright 645 | |
Havcr2 | |
CD366; FLJ14428; Havcr2; HAVcr-2; Hepatitis A virus cellular receptor 2; hepatitis A virus cellular receptor 2 homolog; kidney injury molecule-3; KIM-3; sCD366; soluble CD366; soluble TIM 3; T cell immunoglobulin mucin 3; T cell immunoglobulin mucin-3; T-cell immunoglobulin and mucin domain containing 3; T-cell immunoglobulin and mucin domain-containing protein 3; T-cell immunoglobulin mucin family member 3; T-cell immunoglobulin mucin receptor 3; T-cell membrane protein 3; Tim3; TIM-3; TIMD3; TIMD-3 | |
Mouse | |
100 Tests | |
Primary | |
Human | |
Antibody | |
IgG1 κ |