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CD335 (NKp46), Super Bright 600, clone: 29A1.4, eBioscience™

Rat Monoclonal Antibody

Marke:  Invitrogen 63-3351-80

Additional Details : Gewicht : 0.01000kg

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Artikelnummer. 15815603

  • 159.00 CHF / 25 Mikrogramm


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von Anbieter 31-01-2019
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Beschreibung

Beschreibung

Description: The monoclonal antibody 29A1.4 recognizes mouse NKp46, also known as CD335. CD335, a member of the natural cytotoxicity receptor (NCR) family, is a glycoprotein with 2 Ig-like domains and a short cytoplasmic tail. Expression of CD335 is uniquely found on NK cells (including immature NK cells, defined as DX5- CD3-, and thereby allowing discrimination between NKT cells and NK cells (NKp46+, CD3-). Furthermore, unlike many of the NK markers which also stain NKT cells, staining with 29A1.4 is not strain specific. Staining has been shown on C57Bl/6, SJL, CBA/CA and BALB/C strains. NKp46 has been shown to play a role in NK cell-mediated lysis of several tumor cells and pathogen-infected cell lines.The 29A1.4 monoclonal antibody has been shown to activate NK cells in vitro. The 29A1.4 monoclonal antibody does not deplete NK cells in vivo.Applications Reported: This 29A1.4 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 29A1.4 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 0.5μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Super Bright 600 is a tandem dye that can be excited with the violet laser line (405nm) and emits at 600nm. We recommend using a 610/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (Product # SB-4400) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100μL of cell sample + 100μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 405nm; Emission: 600nm; Laser: Violet Laser
Spezifikation

Spezifikation

CD335 (NKp46)
Monoclonal
0.2mg/mL
PBS with BSA and 0.09% sodium azide; pH 7.2
Q8C567
Ly94; Ncr1; NKp46; MAR1
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse
Flow Cytometry
29A1.4
Super Bright 600
Ncr1
Liquid
Ncr1
IgG2a, kappa
25μg
4°C, store in dark, DO NOT FREEZE!
Primary
17086
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