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CD278 (ICOS), Super Bright 436, clone: ISA-3, eBioscience™

Mouse Monoclonal Antibody

Marke:  Invitrogen 62-9948-41

Additional Details : Gewicht : 0.01000kg

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Artikelnummer. 15815543

  • 158.00 CHF / 25 Stück


Geschätzter Ausliefertermin
von Anbieter 26-02-2019
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Beschreibung

Beschreibung

Description: The ISA-3 monoclonal antibody reacts with human CD278 also known as Inducible Costimulatory molecule (ICOS), H4, CRP-1 and AILIM. ICOS is a T cell specific activation molecule and a third member of the CD28/CTLA-4 family. Human ICOS has a relative molecular mass of 55-60 kDa, composed of 27kDa and 29kDa chains. Human ICOS on activated T cells has potent costimulatory activity for T cell activation and is required for humoral immune responses, in particular for memory B cell and plasma cell generation. ICOS binds to its ligand, B7h/B7RP-1 expressed on activated APCs (antigen presenting cells) and on a number of inflamed peripheral tissues. Plate-bound ISA-3 is costimulatory for T cells and induces production of IL-4, IL-5, IL-10 and other cytokines, but not IL-2. ISA-3 has the same reactivity pattern and characteristics as F44. ISA-3 was generated against the human ICOS antigen. C398.4A, anti-mouse ICOS/H4 (cat. 14-9949), was shown to cross-react with human ICOS but binds to an epitope different from ISA-3. C398.4A stains activated cells brighter than ISA-3; however, it also exhibits higher staining of non-activated human peripheral blood or isolated PBMC. To achieve the brightest staining of ICOS on activated human T cells, please use 13-9948 or 12-9948 rather than 11-9948.Applications Reported: This ISA-3 antibody has been reported for use in flow cytometric analysis.Applications Tested: This ISA-3 antibody has been pre-titrated and tested by flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5μL (0.25μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (cat. SB-4400) to minimize any non-specific polymer interactions.Excitation: 405 nm; Emission: 436 nm; Laser: Violet Laser
Spezifikation

Spezifikation

CD278 (ICOS)
Monoclonal
5μl/Test
PBS with BSA and 0.09% sodium azide; pH 7.2
Q9Y6W8
ICOS
IgG1, kappa
25 Tests
4°C, store in dark, DO NOT FREEZE!
Primary
29851
Flow Cytometry
ISA-3
Super Bright 436
ICOS
Liquid
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Human
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