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CD278 (ICOS) Mouse anti-Human, Biotin, Clone: ISA-3, eBioscience™

Mouse Monoclonal Antibody

Marke:  Affymetrix eBioscience 13-9948-82

Additional Details : Gewicht : 0.25000kg

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Artikelnummer. 15320130

  • 363.00 CHF / 100 Mikrogramm


Verfügbar ab
20-04-2021
Zum Warenkorb hinzufügen

Beschreibung

Beschreibung

Description: The ISA-3 monoclonal antibody reacts with human ICOS (Inducible COStimulatory molecule), also known as H4, CRP-1 and AILIM. ICOS is a T cell specific activation molecule and a third member of the CD28/CTLA-4 family. Human ICOS has a relative molecular mass of 55-60 kDa, composed of 27 kDa and 29 kDa chains. Human ICOS on activated T cells has potent costimulatory activity for T cell activation and is required for humoral immune responses, in particular for memory B cell and plasma cell generation. ICOS binds to its ligand, B7h/B7RP-1 expressed on activated APCs (antigen presenting cells) and on a number of inflamed peripheral tissues. Plate-bound ISA-3 is costimulatory for T cells and induces production of IL-4, IL-5, IL-10 and other cytokines, but not IL-2. ISA-3 has the same reactivity pattern and characteristics as F44. ISA-3 was generated against the human ICOS antigen. C398.4A, anti-mouse ICOS/H4 (cat. 14-9949-82), was shown to cross-react with human ICOS but binds to an epitope different from ISA-3. C398.4A stains activated cells brighter than ISA-3; however, it also exhibits higher staining of non-activated human peripheral blood or isolated PBMC. To achieve the brightest staining of ICOS on activated human T cells, please use 13-9948-82 or 12-9948-42 rather than 11-9948-42. Applications Reported: The ISA-3 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The ISA-3 antibody has been tested by flow cytometric analysis of unstimulated and CD3/CD28-stimulated (3 days) human blood cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filtration: 0.2 μm post-manufacturing filtered. CD278 belongs to the CD28 and CTLA-4 cell-surface receptor family and is highly expressed in activated T-cells. It is essential both for efficient interaction between T and B-cells and for normal antibody responses to T-cell dependent antigens. It enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells.
Spezifikation

Spezifikation

CD278 (ICOS)
Monoclonal
0.5 mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
Q9Y6W8
AILIM, CD278, CVID1, activation-inducible lymphocyte immunomediatory molecule, inducible T-cell costimulator, inducible costimulator
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
ISA-3
Biotin
ICOS
Liquid
ICOS
IgG1, kappa
100 μg
4° C, do not freeze
Primary
29851
Videos
Sicherheitsdatenblatt (SDS)
Dokumentation

For Research Use Only.