CD273 (B7-DC) Monoclonal Antibody (TY25), eBioscience™, Invitrogen™

Beschreibung

Description: The TY25 monoclonal antibody reacts with mouse B7-DC/PD-L2. B7-DC, a member of the B7 family, has a predicted molecular weight of ∽25 kDa and belongs to the Ig superfamily. The mouse B7-DC has a short cytoplasmic tail (4aa). B7-DC is primarily expressed by sub-populations of dendritic cells, monocytes and macrophages. Although B7-DC has structural and sequence similarities to the B7 family, it does not bind CD28/CTLA-4, but binds PD-1. The interactions between PD-1 and B7-DC/PD-L2 have been reported to be involved in costimulation or suppression of T cell proliferation depending on state of cellular activation. TY25 is a useful tool to study the exact function of B7-DC/PD-L2 in APC/T cell interaction and to characterize the expression pattern of this molecule in mouse. Applications Reported: The TY25 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB), and immunohistochemical staining of frozen tissue sections. Immunoblotting should be carried out under non-reducing conditions. It has also been reported for inhibition of ligand binding. (Please use Functional Grade purified TY25, cat. 16-5986, in functional assays.). Applications Tested: The TY25 antibody has been tested by flow cytometric analysis of mouse splenocytes and B7-DC transfected cells. This can be used at less than or equal to 0.5 μg per test.

A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered. Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by co gnate peptides bound to MHC molecules on antigen-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2, both of which are thought act as a negative regulator of T cell activation. However, it has been suggested that PDL-2 can act to stimulate an immunogenic response through and alternative receptor from PD-1. At least three isoforms of PDL-2 are known to exist; this antibody is specific to the largest isoform. PDL-2 antibody has no cross-reactivity to PDL-2.