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CD223 (LAG-3) Monoclonal Antibody (3DS223H), Super Bright™ 645, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
433.00 CHF
Spezifikation
Antigen | CD223 (LAG-3) |
---|---|
Klon | 3DS223H |
Konzentration | 5 μL/Test |
Anwendungen | Flow Cytometry |
Klassifikation | Monoclonal |
Beschreibung
Description: The J105 monoclonal antibody reacts with the human PD-1 (programmed death-1), a 55 kDa member of the CD28 immunoglobulin superfamily. PD-1 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) and plays a key role in peripheral tolerance and autoimmune disease. PD-1 is expressed predomitly on activated T and B lymphocytes. Two novel members of the B7 family have been identified as the PD-1 ligands, PD-L1 (B7-H1) and PD-L2 (B7-DC). Evidence reported to date suggests overlapping functions for these two PD-1 ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. Costaining experiments suggest that eBioJ105 recognizes a different epitope than MIH4 (cat. 11-9969). Applications Reported: This eBioJ105 (J105) antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBioJ105 (J105) antibody has been pre-titrated and tested by flow cytometic analysis of PHA stimulated human peripheral blood cells. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Super Bright 645 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 645 nm. We recommend using a 660/20 bandpass filter.
LAG-3 is a 70-kDa surface glycoprotein belonging to the Ig superfamily with homology to CD4. LAG-3 binds to MHC class II with higher affinity than CD4 and is thought to be involved in the negative regulation of T cell activation and homeostatic proliferation. Surface expression of LAG-3 has been reported on activated T cells (including regulatory T cells) and NK cells. CD8+ T cells usually express LAG-3 at significantly higher levels than CD4+ T cells. Coexpression of LAG-3 and CD49b has been proposed to identify human and mouse Type 1 regulatory T cells (Tr1 cells).Spezifikation
CD223 (LAG-3) | |
5 μL/Test | |
Monoclonal | |
Liquid | |
RUO | |
P18627 | |
LAG3 | |
Primary | |
4° C, store in dark, DO NOT FREEZE! | |
LAG3 |
3DS223H | |
Flow Cytometry | |
Super Bright 645 | |
Mouse | |
Human | |
3902 | |
IgG1 κ | |
Affinity chromatography | |
Antibody |