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CD170 (Siglec F), Super Bright 645, clone: 1RNM44N, eBioscience™

Rat Monoclonal Antibody

Marke:  Invitrogen 64-1702-80

Additional Details : Gewicht : 0.01000kg

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Artikelnummer. 15805683

  • 165.00 CHF / 25 Mikrogramm


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von Anbieter 04-03-2019
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Beschreibung

Beschreibung

Description: This 1RNM44N monoclonal antibody recognizes mouse CD170, also known as Siglec F. Siglec F is a cell surface lectin belonging to the Ig superfamily that binds glycoconjugates containing sialic acids that are commonly found on various cell types. The cytoplasmic domain of Siglec F contains an immunoreceptor tyrosine-based inhibitory motif (ITIM) that initiates intracellular signaling upon ligand binding. Siglec F is expressed mostly on eosinophils and alveolar macrophages and lower levels of this receptor have also been reported on immature myelomonocytic cells. Mouse Siglec F is a functional ortholog of human Siglec 8, however, unlike human Siglec 8, mouse Siglec F is not expressed on mast cells.This 1RNM44N antibody will recognize a formaldehyde-fixed epitope.Applications Reported: This 1RNM44N antibody has been reported for use in flow cytometric analysis.Applications Tested: This 1RNM44N antibody has been tested by flow cytometric analysis of thioglycolate-elicited peritoneal macrophages. This may be used at less than or equal to 0.125μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Super Bright 645 is a tandem dye that can be excited with the violet laser line (405nm) and emits at 645nm. We recommend using a 660/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (Product # SB-4400) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100μL of cell sample + 100μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 405nm; Emission: 645nm; Laser: Violet Laser
Spezifikation

Spezifikation

CD170 (Siglec F)
Monoclonal
0.2mg/mL
PBS with BSA and 0.09% sodium azide; pH 7.2
Q920G3
mSiglec-F; Siglec5; Siglecf
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse
Flow Cytometry
1RNM44N
Super Bright 645
Siglecf
Liquid
Siglecf
IgG2a, kappa
25μg
4°C, store in dark, DO NOT FREEZE!
Primary
233186
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