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CD170 (Siglec F), Super Bright 645, clone: 1RNM44N, eBioscience™

Rat Monoclonal Antibody

Marke:  Invitrogen 64-1702-80

Additional Details : Gewicht : 0.01000kg

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Artikelnummer. 15805683

  • 165.00 CHF / 25 Mikrogramm


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von Anbieter 08-05-2019
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Beschreibung

Beschreibung

Super Bright 645 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 645 nm. We recommend using a 660/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome. When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (Product No. SB-4400) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (Product No. 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product No. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 405 nm; Emission: 645 nm; Laser: Violet Laser SIGLEC5 is a putative adhesion molecule that mediates sialic-acid dependent binding to cells. This protein binds equally to alpha2,3-linked and alpha2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. This molecule is expressed by monocytic/myeloid lineage cells. SIGLEC5 is found at high levels in peripheral blood leukocytes, spleen, bone marrow and at lower levels in lymph node, lung, appendix, placenta, pancreas and thymus. It is expressed by monocytes and neutrophils but absent from leukemic cell lines representing early stages of myelomonocytic differenciation.
Spezifikation

Spezifikation

CD170 (Siglec F)
Monoclonal
0.2 mg/mL
PBS with BSA and 0.09% sodium azide; pH 7.2
Q920G3
mSiglec-F; Siglec5; Siglecf
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse
Flow Cytometry
1RNM44N
Super Bright 645
Siglecf
Liquid
Siglecf
IgG2a, kappa
25 μg
4° C, store in dark, DO NOT FREEZE!
Primary
233186
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