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CD166 (ALCAM) Mouse anti-Human, Super Bright 645, Clone: 3A6, eBioscience™

Mouse Monoclonal Antibody

Marke:  Invitrogen 64-1668-41

Additional Details : Gewicht : 0.01000kg

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Artikelnummer. 15983634

  • 157.00 CHF / 25 Stück


Verfügbar ab
08-10-2020
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Beschreibung

Beschreibung

Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Super Bright 645 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 645 nm. We recommend using a 660/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome. When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product No. SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (Product No. 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product No. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 405 nm; Emission: 645 nm; Laser: Violet Laser Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company. This gene encodes activated leukocyte cell adhesion molecule (ALCAM), also known as CD166 (cluster of differentiation 166), which is a member of a subfamily of immunoglobulin receptors with five immunoglobulin-like domains (VVC2C2C2) in the extracellular domain. This protein binds to T-cell differentiation antigene CD6, and is implicated in the processes of cell adhesion and migration. Multiple alternatively spliced transcript variants encoding different isoforms have been found.
Spezifikation

Spezifikation

CD166 (ALCAM)
Monoclonal
5 μL/Test
PBS with BSA and 0.09% sodium azide; pH 7.2
Q13740
MEMD
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
3A6
Super Bright 645
ALCAM
Liquid
ALCAM
IgG1, kappa
25 Tests
4° C, store in dark, DO NOT FREEZE!
Primary
214
Videos
Dokumentation