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CD16 Mouse anti-Human, Super Bright 645, Clone: eBioCB16 (CB16), eBioscience™

Mouse Monoclonal Antibody

Marke:  Invitrogen 64-0168-42

Additional Details : Gewicht : 0.01000kg

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Artikelnummer. 15815643

  • 329.00 CHF / 100 Stück


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von Anbieter 11-07-2019
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Beschreibung

Beschreibung

It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Super Bright 645 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 645 nm. We recommend using a 660/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome. When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (Product No. SB-4400) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (Product No. 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product No. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 405 nm; Emission: 645 nm; Laser: Violet Laser CD16 (FCGR3A) is a 50-65 kDa cell surface molecule that exists in two forms - a transmembranous form expressed by NK cells and some T cells, and a phosphatidylinositol linked form expressed by granulocytes. CD16 is a low affinity receptor for IgG (FcR III), and is an important receptor mediating ADCC by NK cells. Human CD16 is expressed in two forms – FCGR3A and FCGR3B. FCGR3A is associated with the FcepsilonRI-gamma subunit and is responsible for antibody-dependent NK cell cytotoxicity. Mast cell FCGR3A is associated, with FcepsilonRI-beta subunit. Besides IgG, FCGR3A can be triggered also by oligomeric IgE. FCGR3B is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a pro-adhesive phenotype. Diseases associated with CD16 dysfunction include immunodeficiency 20 and systemic lupus erythematosus.
Spezifikation

Spezifikation

CD16
Monoclonal
5 μL/Test
PBS with BSA and 0.09% sodium azide; pH 7.2
P08637, O75015
FCGR3A, FCGR3B
IgG1, kappa
100 Tests
4° C, store in dark, DO NOT FREEZE!
Primary
2214, 2215
Flow Cytometry
eBioCB16 (CB16)
Super Bright 645
FCGR3A
Liquid
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Human
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