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CD144 (VE-cadherin) Rat anti-Mouse, PerCP-eFluor™ 710, Clone: eBioBV13 (BV13), eBioscience™

Rat Monoclonal Antibody

Marke:  Affymetrix eBioscience 46-1441-82

Additional Details : Gewicht : 0.25000kg

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Artikelnummer. 15331290

  • 378.00 CHF / 100 Mikrogramm


Verfügbar ab
18-05-2021
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Beschreibung

Beschreibung

Description: The BV13 monoclonal antibody reacts with mouse VE-Cadherin (CD144). VE-Cadherin is a 120 kDa member of the type II Cadherin family, characterized by the presence of 5 extracellular cadherin domains (ECD), and anchored to the actin cytoskeleton through their cytoplasmic tail. VE-Cadherin mediates homophilic adhesion between neighbouring endothelial cells and is localized within specialized structures at cell-cell contacts, called adherens junctions. VE-Cadherin is expressed constitutively throughout the entire vasculature, and is required for numerous endothelial cell functions including migration, survival, contact-dependent growth inhibition and endothelial cell assembly into tubular structures. Furthermore, it is thought that VE-Cadherin+CD45- cells from the yolk sac or aorta-gonad-mesonephros (AGM) have the potential to give rise to hematopoietic cells. Applications Reported: This eBioBV13 (BV13) antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBioBV13 (BV13) antibody has been tested by flow cytometric analysis of bEnd.3 cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered. This gene is a classical cadherin from the cadherin superfamily and is located in a six-cadherin cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. The encoded protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, the protein may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. An alternative splice variant has been described but its full length sequence has not been determined.
Spezifikation

Spezifikation

CD144 (VE-cadherin)
Monoclonal
0.2 mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P55284
Cadherin-5, Vascular endothelial cadherin, VE-cadherin, 7B4 antigen, CD144 antigen, CDH5
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse
Flow Cytometry
eBioBV13 (BV13)
PerCP-eFluor™ 710
Cdh5
Liquid
Cdh5
IgG1, kappa
100 μg
4° C, store in dark, DO NOT FREEZE!
Primary
12562
Videos
Sicherheitsdatenblatt (SDS)
Dokumentation

For Research Use Only.