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CD137 (4-1BB), PE-Cyanine7, clone: 4B4 (4B4-1), eBioscience™

Mouse Monoclonal Antibody

Marke:  Invitrogen 25-1379-42

Additional Details : Gewicht : 0.01000kg

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Artikelnummer. 15805373

  • 336.00 CHF / 100 Stück


Geschätzter Ausliefertermin
von Anbieter 04-03-2019
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Beschreibung

Beschreibung

Description: This 4B4 (4B4-1) monoclonal antibody reacts with human CD137 (also known as 4-1BB or TNFRSF9), which is an inducible member of the TNFR family of costimulatory molecules expressed on T cells, natural killer cells, dendritic cells, granulocytes, and mast cells. Involved in recruiting TNFR-associated factors (TRAF) 1 and 2, CD137 signaling plays a role in T cell activation, maintaining the survival of activated and CD8 memory T cells, as well as suppressing myelopoiesis and dendritic cell development. Stimulation of this receptor has also been shown to promote expansion of CD4+CD25+ T regulatory cells i{ex vivoi}. The ligand for CD137, 4-1BBL, is found on activated macrophages, mature B cells, hematopoietic stem cells, and myeloid progenitor cells.Applications Reported: This 4B4 (4B4-1) antibody has been reported for use in flow cytometric analysis.Applications Tested: This 4B4 (4B4-1) antibody has been tested by flow cytometric analysis on two-day Con A-stimulated normal human peripheral blood cells. This can be used at 5μL (0.125μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100μL of cell sample + 100μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561nm; Emission: 775nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Spezifikation

Spezifikation

CD137 (4-1BB)
Monoclonal
5μL/Test
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
Q07011
TNFRSF9
IgG1, kappa
100 Tests
4°C, store in dark, DO NOT FREEZE!
Primary
3604
Flow Cytometry
4B4 (4B4-1)
PE-Cyanine7
TNFRSF9
Liquid
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Human
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