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CD11b Rat anti-Mouse, Super Bright 600, Clone: M1/70, eBioscience™

Rat Monoclonal Antibody

Marke:  Invitrogen 63-0112-82

Additional Details : Gewicht : 0.01000kg

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Artikelnummer. 15983464

  • 353.00 CHF / 100 Mikrogramm


Verfügbar ab
08-10-2020
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Beschreibung

Beschreibung

When using two or more Super Bright dye- conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product No. SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 2-8°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorochrome performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 405 nm; Emission: 600 nm; Laser: Violet Laser Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company. CD11b (integrin alpha-M, ITGAM, integrin alpha-X, ITGAX) is a 165 kDa adhesion molecule that associates non-covalently with integrin beta-2 (CD18). The CD11b/CD18 heterodimeric complex is also known as integrin alpha-M beta-2, Mac-1, and CR3 (complement receptor 3). CD11b is expressed on the surface of monocytes/macrophages, granulocytes, activated lymphocytes, a subset of NK cells, a subset of dendritic cells, and microglia in the brain. CD11b/CD18 functions as the receptor for ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X -these adhesions are critical for cell-cell and cell-matrix interactions. CD11b is expressed on 8% of spleen cells, 44% of bone marrow cells, and less than 1% of thymocytes, and is commonly used as a microglial marker in nervous tissue. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Further, peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages. Diseases associated with CD11b dysfunction include systemic lupus erythematosus 6 and ITGAM-related susceptibility to systemic lupus erythematosus.
Spezifikation

Spezifikation

CD11b
Monoclonal
0.2 mg/mL
PBS with BSA and 0.09% sodium azide; pH 7.2
P05555
Integrin alpha MIntegrin alpha M; HCD11B
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse
Flow Cytometry
M1/70
Super Bright 600
Itgam
Liquid
ITGAM
IgG2b, kappa
100 μg
4° C, store in dark, DO NOT FREEZE!
Primary
16409
Videos
Dokumentation

For Research Use Only