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CD11b, Super Bright 436, clone: M1/70, eBioscience™

Rat Monoclonal Antibody

Marke:  Invitrogen 62-0112-80

Additional Details : Gewicht : 0.01000kg

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Artikelnummer. 15815483

  • 152.00 CHF / 25 Mikrogramm


Geschätzter Ausliefertermin
von Anbieter 31-01-2019
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Beschreibung

Beschreibung

Description: The M1/70 monoclonal antibody reacts with mouse CD11b, the 165-170kDa integrin alphaM. CD11b non-covalently associates with CD18 to form alphaMbeta2 integrin (Mac-1) and binds to CD54 (ICAM-1), C3bi, and fibrinogen. Mac-1 is expressed by macrophages, NK cells, granulocytes, activated lymphocytes and mouse B-1 cells in the peritoneal cavity. M1/70 is also cross-reactive to human CD11b, and can be used for the detection of this antigen on human peripheral blood monocytes, granulocytes, and a subset of NK cells. Through interactions with its ligands, CD11b participates in adhesive cell interactions.Applications Reported: This M1/70 antibody has been reported for use in flow cytometric analysis.Applications Tested: This M1/70 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This may be used at less than or equal to 0.125μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (Product # SB-4400) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.Excitation: 405 nm; Emission: 436 nm; Laser: Violet Laser
Spezifikation

Spezifikation

CD11b
Monoclonal
0.2mg/mL
PBS with BSA and 0.09% sodium azide; pH 7.2
P05555
ly-40
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse
Flow Cytometry
M1/70
Super Bright 436
Itgam
Liquid
ITGAM
IgG2b, kappa
25μg
4°C, store in dark, DO NOT FREEZE!
Primary
16409
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