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Thermo Scientific™ CaptureSelect™ IgG-Fc (Multispecies) Affinity Matrix
Alle ansehen Thermo Scientific ProdukteBeschreibung
CaptureSelect IgG-Fc (Multispecies) Affinity Matrix has been specifically designed to purify IgG through high-affinity binding of the IgG Fc region. This resin can be used for purification of IgG from a broad range of species. The tested species and their specificity are shown in the figure below.
Features of this affinity matrix include:
- One-step IgG purification with high purity, from crude material
- Excellent scalability
- Non-animal-derived
Free of animal components
CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).
Main characteristics:
- Matrix: agarose-based, epoxide activated
- Average particle size: 65 ± 10 μm
- Ligand: CaptureSelect IgG-Fc (multispecies) affinity ligand
- Ligand coupling method: epoxide coupling
- Binding capacity: >25 g IgG/L resin tested with human IgG
- Elution conditions: 0.1 M glycine, pH 3.0
- Flow characteristics: 50–200 cm/h (up to 2 bar)
- Formulation buffer: 20% (v/v) ethanol
Spezifikation
Spezifikation
| Bindungsvermögen | >25 g IgG/L resin |
| Flussrate | 50 to 200 cm/hr. (Up to 2 bar) |
| Matrix | Agarose-based, epoxide activated |
| Partikelgröße | 65 ±10 μm |
| Form | Harzaufschlämmung |
| Produktlinie | CaptureSelect™ |
| Menge | 5 ml |
| Typ | Affinity Matrix |
| Ligandentyp | IgG-Fc (multispecies) affinity ligand |
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