Thermo Scientific™ CaptureSelect™ IgE Affinity Matrix

Features of this affinity matrix include:

• Purification of human IgE, binding different recombinant IgE forms
• Efficient removal of host cell proteins (HCP) and IgE aggregates
• Excellent scalability
• Non–animal-derived

Free of animal components

CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).

Main characteristics

• Matrix: agarose-based, epoxide-activated
• Average particle size: 65 ± 10 μm
• Ligand: CaptureSelect IgE affinity ligand
• Ligand coupling method: aldehyde coupling
• Binding capacity: >5 mg/mL resin
• Elution conditions: 50 mM sodium citrate, 150 mM NaCl, pH 3.5
• Flow characteristics: 50–200 cm/h (up to 2 bar)
• Formulation buffer: 20% (v/v) ethanol