Learn More
Log in om dit product te kopen Registreer vandaag om een webaccount aan te maken
Beschreibung
CaptureSelect HRP Anti-AAVX Conjugate includes a 14-kDa recombinant single domain antibody fragment (VHH affinity ligand) that specifically binds adeno-associated virus particles of multiple AAV serotypes. The antibody fragment binds with high affinity to AAV particles and demonstrates good binding reactivity towards a variety of AAV serotypes, including AAV1 to AAV8, and AAVrh10. The antibody fragment is chemically conjugated to horseradish peroxidase (HRP) via a spacer of appropriate length to maintain full binding capacity of the antibody fragment.
CaptureSelect HRP Anti-AAVX Conjugate can be combined with CaptureSelect Biotin Anti-AAVX Conjugate (Cat. No. 7103522100) in enzyme linked immunoassays to quantify the total number of AAV capsids. Sandwich ELISAs can be designed using the biotin anti-AAVX conjugate as a capture reagent immobilized on a streptavidin coated surface. HRP anti-AAVX conjugate is then used for detection of the bound AAV capsids. The unique binding properties of this pair of anti-AAVX conjugates can circumvent the need for incorporation of different AAV serotype-specific antibodies for AAV assay development.
For assay development to measure AAV9 total capsids, we recommend CaptureSelect Biotin Anti-AAV9 Conjugate (Cat. No. 7103332100) and CaptureSelect HRP Anti-AAV9 Conjugate (Cat. No. 7303332100).
Product details
- Format type: HRP, liquid
- Buffer: PBS, 50% (v/v) glycerol
- Storage: in freezer (-5 to -30°C)
- Concentration: 0.5 mg/mL
- Volume: 0.1 mL
- Recommended working dilution for sandwich ELISA: 1: 5000 to 1: 10,000 (the optimal working dilution should be determined empirically for each application)
Free of animal components
CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).
Spezifikation
Spezifikation
| Konzentration | 0.5 mg/mL |
| Zusammensetzung | Liquid, in PBS (7.4), 50% glycerol |
| Inhalt und Lagerung | Store in freezer (-5°C to -30°C). |
| Form | Flüssig |
| Produktlinie | CaptureSelect |
| Menge | 100 μl |
| Zur Verwendung mit (Anwendung) | Quantitation of multiple AAV capsids |
| Typ | Conjugate |
Indem Sie auf Absenden klicken, erklären Sie sich damit einverstanden, dass Fisher Scientific sich mit Ihnen in Verbindung setzen kann, um Ihr Feedback in diesem Formular zu bearbeiten. Wir werden Ihre Informationen nicht für andere Zwecke weitergeben. Alle bereitgestellten Kontaktinformationen werden in Übereinstimmung mit unserer Datenschutzrichtlinie aufbewahrt. Datenschutzrichtlinie.