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Thermo Scientific™ CaptureSelect™ CH1-XL Magnetic Agarose Beads
Alle ansehen Thermo Scientific ProdukteBeschreibung
CaptureSelect CH1-XL Magnetic Agarose Beads are high-capacity, high-throughput magnetic affinity particles for antibody purification using manual or robotic magnetic separators. The CaptureSelect CH1-XL affinity ligand recognizes the CH1 domain of human IgG, enabling purification of all subclasses (1, 2, 3, and 4) of human IgG and Fab fragments, independent of the light-chain isotype and source material. Due to selectivity for the CH1 domain, no co-purification of free light chains will occur. IgG and Fab fragments can be directly isolated from complex source materials in a single step with high purity and yield.
Features of CaptureSelect CH1-XL Magnetic Agarose Beads include:
- One-step human IgG purification with high purity, from crude material
- Compatible with manual and automated workflows (e.g., Thermo Scientific KingFisher instruments)
- Short operation time
- Mild elution at pH 4
- No sample dilution required during isolation, repetitive sample loading feasible
- No binding to bovine antibodies, allowing use of fetal bovine serum in the sample material
- Non-animal-derived
Free of animal components
CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).
Main characteristics:
- Matrix: 6% cross-linked magnetized agarose
- Average particle size: 25–30 μm
- Suspension: 25% slurry
- Ligand: CaptureSelect CH1-XL affinity ligand
- Binding capacity: >4 mg IgG/mL slurry
- Elution conditions: 50 mM sodium acetate, pH 4.0/0.1 M glycine, pH 3.0
Spezifikation
Spezifikation
| Bindungsvermögen | >4 mg IgG/mL slurry |
| Matrix | 6% cross-linked magnetized agarose |
| Partikelgröße | 25 to 30 μm |
| Form | Resin Slurry |
| Produktlinie | CaptureSelect™ |
| Menge | 1 ml |
| Typ | Magnetische Agarose-Beads |
| Ligandentyp | CH1-XL affinity ligand |
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