missing translation for 'onlineSavingsMsg'
Learn More
Learn More
C32 (human amelanotic melanoma) Whole cell lysate, Non-denatured; Abnova
C32 (human amelanotic melanoma) whole cell lysate
Marke: Abnova L002V2.200ug
Additional Details : Gewicht : 0.02000kg
Beschreibung
- Whole cell lysate (non-denatured)
- Tissue: skin
- Host: Human
- Preparation: homogenization in ice-cold modified RIPA Lysis
- Lysis buffer: 50mM Tris-HCl pH 7.4, 150mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% sodium deoxycholate, 1mM PMSF
- Storage buffer: in modified RIPA lysis buffer
Western blotting, immunoprecipitation
Spezifikation
| Immunoprecipitation, Western Blot | |
| Whole cell lysate (non-denatured) | |
| Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). | |
| 200 μg | |
| Human | |
| Store at -80°C. Aliquot to avoid repeated freezing and thawing. |
| Skin | |
| Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. | |
| 12.5% SDS-PAGE Stained with Coomassie Blue. | |
| In modified RIPA Lysis Buffer. | |
| 5 mg/mL |