Melford Blasticidin S HCl Solution 10 mg/mL

In the life science marketplace, Blasticidin S HCl Solution 10 mg/mL is primarily used as a selection agent in cell culture and molecular biology experiments. It is incorporated into cell culture media to selectively kill cells that do not contain the desired genetic modification or resistance gene. This allows researchers to selectively grow and study cells that have been successfully transfected or transformed with the gene of interest.

Blasticidin S works by inhibiting protein synthesis in both prokaryotic and eukaryotic cells. It blocks the peptidyl transferase activity of the ribosome, leading to the premature termination of protein synthesis and eventual cell death.

This antibiotic is commonly used in the production of stable cell lines expressing recombinant proteins or genes of interest. It provides a means to select and maintain cells that have integrated the desired genetic construct, making it an essential tool in genetic engineering and protein expression studies.

Additionally, Blasticidin S HCl Solution 10 mg/mL finds application in gene editing technologies such as CRISPR-Cas9. It can be used as a selectable marker to identify and isolate cells that have successfully undergone gene editing, facilitating the creation of genetically modified cell lines or organisms.

Supplied as a 10 mg/mL sterile solution in HEPES buffer.

Packaging Options:

B12150-0.1 (100mg) = total 5 x 20 mg

B12150-0.2 (200mg) = total 10 x 20 mg

B12150-0.5 (500mg) = total 25 x 20 mg