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  7. Applied Biosystems™ High-Capacity cDNA Reverse Transcription Kit 

Applied Biosystems™ High-Capacity cDNA Reverse Transcription Kit

Codice prodotto. 10400745 Sfoglia Tutto Applied Biosystems Prodotti
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Kit only
Kit with Rnase Inhibitor
N. di reazioni:
1,000 Reactions
200 Reactions
Dimensione della confezione:
1 set
1000 reazioni
200 reazioni
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Codice prodotto. Include N. di reazioni Dimensione della confezione
10400745 Kit only 200 Reactions 200 reazioni
10249814 Kit with Rnase Inhibitor 200 Reactions 1 set
10186954 Kit only 1,000 Reactions 1000 reazioni
10743647 Kit with Rnase Inhibitor 1,000 Reactions 1 set
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Codice prodotto. 10400745 Fornitore Applied Biosystems™ N. del fornitore 4368814
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Descrizione

The High Capacity cDNA Reverse Transcription Kit, available with or without RNase Inhibitor, contains all components necessary for the quantitative conversion of up to 2 μg of total RNA into single-stranded cDNA in a single 20 μL reaction.

The High Capacity cDNA Reverse Transcription Kit, available with or without RNase Inhibitor, contains all components necessary for the quantitative conversion of up to 2 μg of total RNA into single-stranded cDNA in a single 20 μL reaction. Both types of kit deliver extremely high-quality, single-stranded cDNA from 0.02–2 μg total RNA. Reactions can be scaled up to 100 μL to generate 10 μg of cDNA from a single reaction.

The High Capacity cDNA Reverse Transcription Kit with RNase Inhibitor includes the recombinant enzyme used to prevent degradation of RNA templates and lacks DNA endonuclease activity for better product yield.

In addition to their use in RT-PCR, this kit may be used in traditional PCR, microarrays, and for the generation of cDNA archives.

Features of the High Capacity cDNA Reverse Transcription Kit include:

  • Linear target amplification for real-time PCR
  • Higher yields and precision than other cDNA synthesis kits at a fraction of the cost
  • 10-fold greater dynamic range than other kits

Extensively tested with a variety of templates

Quantitative first-strand synthesis of all RNA species is achieved with the use of the random primers. This kit has been extensively tested with a variety of RNA templates, including GC- and AU-rich targets and RNAs expressed at low levels. cRNA can also be efficiently generated from in vitro transcription of cDNA.

Order Info

Shipping Condition: Dry ice

TRUSTED_SUSTAINABILITY

Specifica

Concentrazione 50 U/μL
Content And Storage

• 10X RT Buffer, 1 mL
• 10X RT Random Primers, 1 mL
• 25X dNTP Mix, 0.2 mL (100 mM)
• MultiScribe Reverse Transcriptase, 2 x 0.1 mL (50 U/μL)

Store at –15 to –25°C.
Formato Kit
Reaction Speed 120 min.
Tecnica Reverse Transcription
Optimal Reaction Temperature 37°C
Quantità 1 Ea.
Reverse Transcriptase MultiScribe
Ribonuclease H Activity Yes
Shipping Condition Dry Ice
Da utilizzare con (applicazione) RT-PCR
Tipo di prodotto finale First-Strand cDNA
N. di reazioni 200 Reactions
Reaction Format Separate components
Tipo di reagente Reverse Transcription
Dimensioni (prodotto finale) 7 kb or Less
Starting Material RNA
Include Kit only
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For Research Use Only. Not for use in diagnostic procedures.

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