Stat2 Mouse anti-Human, Unlabeled, Clone: 22, BD

Beschreibung

The Stat proteins function as both cytoplasmic signal transducers and activators of transcription. Stat91/84 (the two proteins are the result of alternate splicing-Stat91 has an additional 38 C-terminal amino acids) and Stat113 were the first identified members of this protein family. These three polypeptides contain both SH2 and SH3 domains and have also been described as members of the ISGF3 (interferon-stimulated gene factor 3) complex. With the discovery of additional members of the Stat family (Stats3 & 4), the nomenclature has been revised to indicate the Stat family members in the order of their discovery. Stat 91, 84, and 113 have become Stat1α, Stat1β, and Stat2, respectively. In response to IFN-α treatment, Stat1α, Stat1β, and Stat2 become tyrosine-phosphorylated and migrate to the nucleus where they join a 48kDa DNA binding protein and subsequently direct the transcription at IFN-α responsive elements. The genes encoding Stat2 and Stat1 are closely related. An extended acidic C-terminal region exclusive to Stat2 implicates a potential role in gene activation. Stat2 phosphorylation can occur independently of Stat1α, but phosphorylated Stat2 does appear to be dependent on its interaction with Stat1α for efficient nuclear localization and/or stability.

Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blotting