missing translation for 'onlineSavingsMsg'
Learn More

CD25 Rat anti-Mouse, PE-CF594, Clone: PC61, BD

Rat Monoclonal Antibody

Marke:  BD Biosciences 562695

Additional Details : Gewicht : 0.00820kg

 Lernen Sie weitere Varianten dieses

Artikelnummer. 15832159

  • 192.00 CHF / 1 Stück


Verfügbar ab
01-06-2021
Zum Warenkorb hinzufügen

Beschreibung

Beschreibung

The PC61 antibody reacts with CD25, the low-affinity IL-2 Receptor α chain (IL-2Rα, p55) expressed on activated T and B lymphocytes from all mouse strains tested. IL-2Rα by itself is not a signaling receptor. However, it can combine with IL-2 Receptor β (CD122) and γc (CD132) chains to form high-affinity, signaling receptor complexes for IL-2. Resting T and B lymphocytes and resting and activated NK cells do not express IL-2Rα. CD25 is transiently expressed at a low level during normal B-cell development in the bone marrow on the CD45R/B220low TdT- sIg- Pre-B/Pre-B-II and CD45R/B220low TdT- sIgM+ sIgD- immature B stages, but not on the CD45R/B220low TdT+ sIg- Pro-B/Pre-B-I stage nor on CD45R/B220high TdT- sIgM+ sIgD+ mature B cells. It is expressed at a higher level during a very early stage of T-cell development in fetal and adult thymus. Peripheral CD25+CD4+ lymphocytes called regulatory T (Treg) cells are involved in the maintenance of self-tolerance. It has also been reported that dendritic cells express CD25, recognized by mAb 7D4 (Cat. No. 553068). The PC61 antibody recognizes an epitope of CD25 which is distinct from the IL-2 binding site and from those recognized by mAbs 3C7 (Cat. No. 557364) and 7D4 (Cat. No. 553068). It blocks binding of IL-2 to CD25, presumably by inducing a conformational change in CD25.

This antibody is conjugated to BD Horizon™ PE-CF594, which has been developed exclusively by BD Biosciences as a better alternative to PE-Texas Red™. PE-CF594 excites and emits at similar wavelengths to PE-Texas Red™ yet exhibits improved brightness and spectral characteristics. Due to PE having maximal absorption peaks at 496nm and 564nm, PE-CF594 can be excited by the blue (488-nm), green (532-nm) and yellow-green (561-nm) lasers and can be detected with the same filter set as PE-Texas Red™ (eg 610/20-nm filter).

Spezifikation

Spezifikation

CD25
PC61
PE-CF594
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Rat
IgG1, λ
RUO
Primary
Murine
Flow Cytometry
0.2mg/mL
Interleukin-2 receptor alpha chain; IL-2RA; IL-2Rα; Il2ra; IL-2R p55
Affinity Purified
IL-2-dependent cytolytic mouse T-cell clone B6.1
25μg
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Monoclonal
Videos
Sicherheitsdatenblatt (SDS)
Dokumentation

For Research Use Only.