Invitrogen™ Human alpha-1 PDX Recombinant Protein

Beschreibung

alpha-1 PDX Recombinant Protein, RP-070, from Invitrogen.

Alpha1-PDX is a potent and selective inhibitor of furin (Ki = 0.6 nM). Alpha1-PDX was engineered by the construction of a variant of alpha1-antitrypsin that contains a minimal furin consensus sequence, RXXR. Cellular expression of alpha1-PDX effectively blocks the furin-dependent processing of protein precursors. In addition, extracellular application of alpha1-PDX can also block the processing of furin substrates. Alpha1-PDX has been shown to inhibit the processing of biologically significant proteins including many viral envelope glycoproteins, bacterial toxins, receptors and growth factors. RP-070 was obtained from E. coli transformed with the alpha1-PDX gene. RP-070 can be used in furin inhibition assays. For furin inhibition assays, cells should be treated with 8.0-20μM alpha1-PDX for 1 hour (see above references). For in vitro assays, 0.1-2.0 μM alpha1-PDX should be used. Purity: >80% (most lots are 80-95%) NOTE: Freezing this product may result in damage of the protein. NOTE: Once rehydrated, this protein retains optimal reactivity for 2-4 days at 4 degrees C. Longer storage time can be achieved by storing in a serum rich medium. RP-070 can be used for protein biochemical, intracellular trafficking, and protein kinetic assays. Molecular weight is approximately 46.7 kDa.