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Applied Biosystems™ AgPath-ID™ One-Step RT-PCR Reagents with manual
Alle ansehen Applied Biosystems ProdukteBeschreibung
- Recommended for RNA pathogen amplification
- Consistent amplification of RNA targets with high specificity and sensitivity
- Optimized to work with your target-specific primers and probes
- Contains ROX for quantitative fluorescent signal normalization
AgPath-ID™ One-Step RT-PCR Reagents are configured for fast and simple reaction setup. The reactions are assembled in a single tube, minimizing sample handling errors and expediting set-up time. Once assembled, results are available in approximately one hour. The 25X RT-PCR Enzyme Mix included in the kit contains highly efficient ArrayScript™ Reverse Transcriptase, a mutant MMLV RT that produces high cDNA yields, and AmpliTaq Gold™ polymerase, the preferred hot-start DNA polymerase for specific target amplification. The 2X RT-PCR Buffer has been optimized for efficient, robust reverse transcription and PCR includes the passive reference dye, ROX™ Dye, for quantitative fluorescent signal normalization.
Spezifikation
Spezifikation
| Konzentration | 25X |
| Inhalt und Lagerung | Sufficient for 1000 reactions of 25 μL, contains: • 14 mL 2 x RT-PCR Buffer • 1100 μL 25 x RT-PCR Enzyme Mix • 25 mL Nuclease-free Water. |
| Beschreibung | Works on all AB platforms, but is optimized for the 7500. |
| Nachweisverfahren | Primer-Sonde |
| Format | Tube |
| GC-Rich PCR Performance | Hoch |
| PCR-Methode | 1-step RT-qPCR |
| Polymerase | AmpliTaq Gold DNA-Polymerase |
| Reaktionsgeschwindigkeit | Standard |
| Zur Verwendung mit (Geräte) | 7500 System |
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Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.
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