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  7. Applied Biosystems™ AgPath-ID™ One-Step RT-PCR Reagents

Applied Biosystems™ AgPath-ID™ One-Step RT-PCR Reagents

Codice prodotto. 30057768 Sfoglia Tutto Applied Biosystems Prodotti
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N. di reazioni:
100 Reactions
1000 Reactions
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Dimensione della confezione:
100 reazioni
1000 reazioni
500 reazioni
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Codice prodotto. N. di reazioni Dimensione della confezione
30057768 100 Reactions 100 reazioni
30057790 500 Reactions 500 reazioni
30057794 1000 Reactions 1000 reazioni
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Codice prodotto. 30057768 Fornitore Applied Biosystems™ N. del fornitore AM1005
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Descrizione

AgPath-ID™ One-Step RT-PCR Reagents are designed for sensitive, robust amplification of RNA targets using a rapid single-tube TaqMan™ real-time reverse transcription PCR (RT-PCR) strategy.

AgPath-ID™ One-Step RT-PCR Reagents are designed for sensitive, robust amplification of RNA targets using a rapid single-tube TaqMan™ real-time reverse transcription PCR (RT-PCR) strategy.

  • Recommended for RNA pathogen amplification
  • Consistent amplification of RNA targets with high specificity and sensitivity
  • Optimized to work with your target-specific primers and probes
  • Contains ROX for quantitative fluorescent signal normalization

AgPath-ID™ One-Step RT-PCR Reagents are configured for fast and simple reaction setup. The reactions are assembled in a single tube, minimizing sample handling errors and expediting set-up time. Once assembled, results are available in approximately one hour. The 25X RT-PCR Enzyme Mix included in the kit contains highly efficient ArrayScript™ Reverse Transcriptase, a mutant MMLV RT that produces high cDNA yields, and AmpliTaq Gold™ polymerase, the preferred hot-start DNA polymerase for specific target amplification. The 2X RT-PCR Buffer has been optimized for efficient, robust reverse transcription and PCR includes the passive reference dye, ROX™ Dye, for quantitative fluorescent signal normalization.

TRUSTED_SUSTAINABILITY

Specifica

Concentrazione 25X
Content And Storage Sufficient for 100 reactions of 25 μL, contains:
• 1375μL 2X RT-PCR Buffer
• 110μL 25X RT-PCR Enzyme Mix
• 1.75mL Nuclease-free Water.
Descrizione Works on all AB platforms, but is optimized for the 7500.
Metodo di rilevamento Primer-probe
Formato Tube
GC-Rich PCR Performance High
PCR Method 1-step RT-qPCR
Polimerasi AmpliTaq Gold DNA Polymerase
Reaction Speed Standard
Da utilizzare con (apparecchiatura) 7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex, QuantStudio™ Absolute Q Digital PCR System
Passive Reference Dye ROX (Pre-mixed)
Linea di prodotti AgPath-ID, Ambion
Product Type One-Step RT-PCR Reagent
Quantità 100 reactions
Reverse Transcriptase ArrayScript™
Shipping Condition Dry Ice
Sufficiente per 100 Reactions
Fedeltà (rispetto a taq) 25 X
N. di reazioni 100 Reactions
Tipo di campione RNA
Volume 1.75 mL
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