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Abnova bmMSCs(Human bone marrow-derived mesenchymal stem cell) whole cell lysate (nondenatured)
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Menge:
50 μg
Packungsgröße:
50 Mikrogramm
Beschreibung
- Stem cells maintained in the undifferentiated state using specialized culturing protocols
- Monitored for undifferentiated cell morphology
- Analyzed using Flow Cytometry against specific cell marker
- Coommasie Blue Staining of total cell lysate in SDS-PAGE
Western Blotting, Immunoprecipitation
Spezifikation
Spezifikation
| Zur Verwendung mit (Anwendung) | Immunoprecipitation, Western Blot |
| Tissue | Knochenmark |
| Beschreibung | Whole cell lysate (non-denatured) |
| Lysepuffer | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. |
| Vorbereitungsmethode | Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). |
| Qualitätskontrollen | 12.5% SDS-PAGE Stained with Coomassie Blue. |
| Menge | 50 μg |
| Lagerungspuffer | In modified RIPA Lysis Buffer. |
| Wirtsspezies | Human |
| Konzentration | 4 mg/mL |
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