Gibco™ AAV-MAX Helper-Free AAV Production System Kit, without lysis buffer

• A scalable and high-titer platform for suspension AAV production
• Based on a high-density suspension culture of clonal 293F-derived Viral Production Cells 2.0 in Viral Production Medium
• Optimal viral titers are mediated by our advanced lipid nanoparticle transfection reagent in combination with a novel production enhancer
• The components are designed to work synergistically, resulting in maximal titers and eliminating the need to optimize reagents and protocols
• Note, this version of the kit does not contain the AAV-MAX Lysis Buffer
• To purchase the kit including the AAV-MAX Lysis Buffer, please see A51217
• All components are also available for purchase separately

Features of the AAV-MAX Helper-Free AAV Production System include:

• Components work synergistically to produce high AAV titers greater than 5x10¹⁰ vg/mL (unpurified)
• Suspension-based system has scalable protocols from shake flask to bioreactor scale
• Simplified workflow—streamlined protocol using helper virus-free triple transfection
• Clonal, 293F-derived, high-producer cell line
• Robust transfection protocols are adaptable to large scale transfection

Components of the AAV-MAX System Kit include:

• 1 vial of Viral Production Cells 2.0
• 1 L of Viral Production Medium
• 1 AAV-MAX Transfection Kit for 1 L of production
• 100 mL Viral-Plex Complexation Buffer
• 100 mL GlutaMAX™ Supplement