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Thermo Scientific™ 1-Step™ TMB-Blotting Substrate Solution

Product Code. 10066433 Shop All Thermo Scientific Products
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250 mL
Unit Size:
250mL
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Product Code. Quantity Unit Size
10066433 250 mL 250mL
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Product Code. 10066433 Supplier Thermo Scientific™ Supplier No. 34018
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Description

Ready-to-use blue TMB peroxidase (HRP) substrate specially formulated for chromogenic detection in Western blot and IHC experiments.

Thermo Scientific™ Pierce TMB-Blotting 1-Step Solution is a ready-to-use solution of the blue TMB peroxidase (HRP) substrate that is specially formulated for chromogenic detection in Western blotting and immunohistochemistry methods.

TMB (3,3',5,5'-tetramethylbenzidine; MW = 240.4) is most often used as a soluble substrate to detect the activity of peroxidase-conjugated antibodies in ELISA. Pierce TMB-Blotting Solution is formulated to precipitate and localize the blue reaction product onto membrane surfaces or tissue samples at sites where the peroxidase-substrate reaction occurs. TMB-Blotting Substrate produces a very large signal-to-noise ratio, although its high sensitivity requires careful washing and blocking to avoid background staining. When performing immunohistochemical techniques, aqueous counterstains and mounting medium must be used.

Highlights:

  • HRP substrate – for sensitive detection of horseradish peroxidase activity on solid media

  • Chromogenic – no special equipment needed for visualization; produces dark blue bands in Western blots; is not as susceptible to fading as AEC or 4-CN substrates

  • Ready-to-use – no organic solvents are required to dissolve; no dilution necessary for use

  • Complete – does not require other buffers or addition of hydrogen peroxide

TRUSTED_SUSTAINABILITY

Specifications

Product Type Substrate
Detection Method Colorimetric
Form Liquid
Product Line 1-Step
Quantity 250 mL
Substrate Horseradish Peroxidase
Reactivity Horseradish Peroxidase
Content And Storage Upon receipt store product at 4°C. Product is shipped at ambient temperature.

Safety and Handling

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Product Identifier
  • 1-Step TMB-Blotting, 250 mL
 Signal Word
  • Warning
 Hazard Category
  • Acute toxicity Category 4
  • Specific target organ toxicity Category 2
 Hazard Statement
  • H302-Harmful if swallowed.
  • H371-May cause damage to organs.
 Precautionary Statement
  • P270-Do not eat, drink or smoke when using this product.
  • P260-Do not breathe dust/fume/gas/mist/vapours/spray.
  • P264-Wash thoroughly after handling.
  • P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
  • P330-Rinse mouth.
  • P308+P311-IF exposed or concerned: Call a POISON CENTER/doctor/.
 Supplemental information
  • MIXTURE LIST-Contain: Methyl alcohol

For Research Use Only. Not for use in diagnostic procedures.

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